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BD Pharmingen- MiCK-1 Mouse Cytokine Positive Control Cells_BD Pharmingen

艾美捷科技 • 2025年08月22日 13:05:27 • 抗体 • 阅读 20 • 标签
产品信息
简单描述
This suspension contains Mouse intracellular CytoKine-1 (MiCK-1) Positive Control Cells. The MiCK-1 cell suspension contains ~5 x 10^6 fixed, non-permeabilized mouse lymphoid cells . The suspension includes cells that express easily detectable levels of intracellular IL-2, TNF (also known as TNF-α), and IFN-γ as determined by immunofluorescent intracellular cytokine staining and flow cytometry. MiCK-1 cell suspensions were prepared by stimulating mouse spleen cells in the presence of a protein transport inhibitor. After stimulation, the cells were harvested and were incubated with Fc Block™ [rat IgG2b,κ anti-mouse CD16/CD32 (FcγII/III receptor) antibody; Cat. No. 553142] to reduce Fc receptor-mediated background staining. The cells were fixed and then stored in 10% dimethylsulfoxide and 90% fetal bovine serum at -80°C.  Each lot of MiCK-1 contains a measurable proportion of cytokine-producing cells.  Representative flow cytometric results are shown for typical staining of MiCK-1 cells. Data from individual lots of MiCK-1 cells may vary. Investigators should anticipate similar (though not identical) results to those shown due to differences in staining methodology and in flow cytometers/cytometer settings.
商品描述
This suspension contains Mouse intracellular CytoKine-1 (MiCK-1) Positive Control Cells. The MiCK-1 cell suspension contains ~5 x 10^6 fixed, non-permeabilized mouse lymphoid cells . The suspension includes cells that express easily detectable levels of intracellular IL-2, TNF (also known as TNF-α), and IFN-γ as determined by immunofluorescent intracellular cytokine staining and flow cytometry. MiCK-1 cell suspensions were prepared by stimulating mouse spleen cells in the presence of a protein transport inhibitor. After stimulation, the cells were harvested and were incubated with Fc Block™ [rat IgG2b,κ anti-mouse CD16/CD32 (FcγII/III receptor) antibody; Cat. No. 553142] to reduce Fc receptor-mediated background staining. The cells were fixed and then stored in 10% dimethylsulfoxide and 90% fetal bovine serum at -80°C.  Each lot of MiCK-1 contains a measurable proportion of cytokine-producing cells.  Representative flow cytometric results are shown for typical staining of MiCK-1 cells. Data from individual lots of MiCK-1 cells may vary. Investigators should anticipate similar (though not identical) results to those shown due to differences in staining methodology and in flow cytometers/cytometer settings.
克隆号
(RUO)
浓度
5x10^6 cells/ml
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
目标/特异性
Mick-1 Cytokine positive control cells
制备和贮存
存储溶液
Frozen in FBS and 10% DMSO.
保存方式
Frozen in FBS and 10% DMSO.
文献
文献
研发参考(2) 1. BD Biosciences. Techniques for Immune Function Analysis, Application Handbook 1st Edition. 2003. Available: http://www.bdbiosciences.com/pdfs/manuals/02-8100055-21A1rr.pdf 2007, Jan. 25. 2. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology).

参考图片

Characteristic staining of MiCK-1 Positive Control Cells with IL-2, TNF, and IFN-γ. MiCK-1 cells were washed, permeabilized, and subsequently stained with PE-rat IgG1 isotype control (PE-R3-34; Cat. No. 554685; see Panel A), PE-rat anti-mouse IL-2 antibody (PE-JES6-5H4, Cat. No. 554428 see Panel B), PE-rat anti-mouse TNF (PE-MP6-XT22, Cat. No. 554419; Panel C), and PE-rat anti-mouse IFN-γ (PE-XMG1.2, Cat. No. 554412; Panel D). Despite fixation and freezing, the side- and forward-scattered light signals for these control cells (see Panel E) remain similar to those for freshly-prepared lymphoid cell preparations (data not shown). Bivariate markers for the gated monocyte population (Panel E) were set based on autofluorescence and isotype controls. The percentage of cytokine expressing cells was calculated based on the gated monocyte cell population shown in Panel E.

Characteristic staining of MiCK-1 Positive Control Cells with IL-2, TNF, and IFN-γ. MiCK-1 cells were washed, permeabilized, and subsequently stained with PE-rat IgG1 isotype control (PE-R3-34; Cat. No. 554685; see Panel A), PE-rat anti-mouse IL-2 antibody (PE-JES6-5H4, Cat. No. 554428 see Panel B), PE-rat anti-mouse TNF (PE-MP6-XT22, Cat. No. 554419; Panel C), and PE-rat anti-mouse IFN-γ (PE-XMG1.2, Cat. No. 554412; Panel D). Despite fixation and freezing, the side- and forward-scattered light signals for these control cells (see Panel E) remain similar to those for freshly-prepared lymphoid cell preparations (data not shown). Bivariate markers for the gated monocyte population (Panel E) were set based on autofluorescence and isotype controls. The percentage of cytokine expressing cells was calculated based on the gated monocyte cell population shown in Panel E.

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