CD3

Mouse BALB/c IgG3, κ

PHA-stimulated rat lymph node and spleen cells

The G4.18 monoclonal antibody specifically recognizes the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. It has been reported that CD3 expression is down-regulated within 24 hours in concanavalin A-stimulated rat T cells, and soluble mAb inhibits the allogeneic mixed-lymphocyte proliferative response and cell-mediated cytotoxicity to allogeneic target cells. In vivo treatment with G4.18 mAb prevents cardiac and skin allograft rejection, resulting in donor-specific tolerance. Pre-incubation of splenocytes with the alternate anti-rat CD3 monoclonal antibody, 1F4, blocks staining with mAb G4.18.

G4.18 The G4.18 monoclonal antibody specifically recognizes the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. It has been reported that CD3 expression is down-regulated within 24 hours in concanavalin A-stimulated rat T cells, and soluble mAb inhibits the allogeneic mixed-lymphocyte proliferative response and cell-mediated cytotoxicity to allogeneic target cells. In vivo treatment with G4.18 mAb prevents cardiac and skin allograft rejection, resulting in donor-specific tolerance. Pre-incubation of splenocytes with the alternate anti-rat CD3 monoclonal antibody, 1F4, blocks staining with mAb G4.18.

Mouse BALB/c IgG3, κ

克隆 G4.18 (RUO)

1.0 mg/ml

Flow cytometry (Routinely Tested), Bioassay (Tested During Development), (Co)-stimulation, Immunohistochemistry-frozen, Immunoprecipitation, Western blot (Reported)

Rat (QC Testing)

CD3

CD3 Complex; T3

No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

研发参考(6) 1. Brenan M, Rees DJ. Sequence analysis of rat integrin alpha E1 and alpha E2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph. Eur J Immunol. 1997; 27(11):3070-3079. (Clone-specific: Immunofluorescence, Western blot). 2. Naper C, Vaage JT, Lambracht D, et al. Alloreactive natural killer cells in the rat: complex genetics of major histocompatibility complex control. Eur J Immunol. 1995; 25(5):1249-1256. (Clone-specific: Cytotoxicity). 3. Nelson DJ, McMenamin C, McWilliam AS, Brenan M, Holt PG. Development of the airway intraepithelial dendritic cell network in the rat from class II major histocompatibility (Ia)-negative precursors: differential regulation of Ia expression at different levels of the respiratory tract. J Exp Med. 1994; 179(1):203-212. (Clone-specific: Immunohistochemistry). 4. Nicolls MR, Aversa GG, Pearce NW, et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.. Transplantation. 1993; 55(3):459-68. (Immunogen: (Co)-stimulation, Flow cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition, Stimulation). 5. Strickland D, Kees UR, Holt PG. Regulation of T-cell activation in the lung: alveolar macrophages induce reversible T-cell anergy in vitro associated with inhibition of interleukin-2 receptor signal transduction. Immunology. 1996; 87(2):250-258. (Biology). 6. Upham JW, Strickland DH, Bilyk N, Robinson BW, Holt PG. Alveolar macrophages from humans and rodents selectively inhibit T-cell proliferation but permit T-cell activation and cytokine secretion. Immunology. 1995; 84(1):142-147. (Biology).