BD Pharmingen- PE Mouse Anti-Rat - T-Cell Receptor_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
TCR αβ
宿主
Mouse BALB/c IgG1, κ
免疫原
Rat T blasts and rat erythrocytes
简单描述
The R73 monoclonal antibody specifically recognizes the αβ T-cell Receptor (TCR) found on most peripheral T lymphocytes, intestinal intraepithelial lymphocytes, and thymocytes. It does not react with γδ TCR-bearing cells. Cross-linked R73 mAb induces T-cell activation and differentiation. In vivo treatment with mAb R73 can suppress immune function of peripheral αβ TCR-expressing T cells, and reduce the severity of experimental autoimmune, transplant rejection, and graft-versus-host responses.
商品描述
R73
The R73 monoclonal antibody specifically recognizes the αβ T-cell Receptor (TCR) found on most peripheral T lymphocytes, intestinal intraepithelial lymphocytes, and thymocytes. It does not react with γδ TCR-bearing cells. Cross-linked R73 mAb induces T-cell activation and differentiation. In vivo treatment with mAb R73 can suppress immune function of peripheral αβ TCR-expressing T cells, and reduce the severity of experimental autoimmune, transplant rejection, and graft-versus-host responses.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 R73 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Rat (QC Testing)
目标/特异性
TCR αβ
背景
别名
TCR α/β: TCR αβ; αβ TCR; alpha beta TCR
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(13)
1. Fangmann J, Schwinzer R, Wonigeit K. Unusual phenotype of intestinal intraepithelial lymphocytes in the rat: predominance of T cell receptor alpha/beta+/CD2- cells and high expression of the RT6 alloantigen. Eur J Immunol. 1991; 21(3):753-760. (Biology).
2. Gaede K, Nazet M, Bosse D, Hunig, Heesemann J. Treatment of arthritis in Lewis rats by a monoclonal antibody against alpha beta T cell receptor: differential sensitivity of Yersinia-induced arthritis versus adjuvant arthritis. Clin Immunol Immunopathol. 1995; 77(3):339-348. (Biology).
3. Hasegawa T, Tanaka T, Yoshikai Y. The appearance and role of gamma delta T cells in the peritoneal cavity and liver during primary infection with Listeria monocytogenes in rats. Int Immunol. 1992; 4(10):1129-1136. (Biology).
4. Heidecke CD, Hancock WW, Jakobs F, et al. alpha/beta-T cell receptor-directed therapy in rat cardiac allograft recipients. Treatment prior to alloantigen exposure prevents sensitization and abrogates accelerated rejection. Transplantation. 1995; 59(1):78-84. (Biology).
5. Heidecke CD, Hancock WW, Westerholt S, et al. alpha/beta-T cell receptor-directed therapy in rat allograft recipients. Long-term survival of cardiac allografts after pretreatment with R73 mAb is associated with upregulation of Th2-type cytokines. Transplantation. 1996; 61(6):948-956. (Biology).
6. Hunig T, Wallny HJ, Hartley JK, Lawetzky A, Tiefenthaler G. A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes. J Exp Med. 1989; 169(1):73-86. (Immunogen).
7. Jung S, Kramer S, Schluesener HJ, Hunig T, Toyka K, Hartung HP. Prevention and therapy of experimental autoimmune neuritis by an antibody against T cell receptors-alpha/beta. J Immunol. 1992; 148(12):3768-3775. (Biology).
8. Kiely PD, Thiru S, Oliveira DB. Inflammatory polyarthritis induced by mercuric chloride in the Brown Norway rat. Lab Invest. 1995; 73(2):284-293. (Biology).
9. Mitnacht R, Tacke M, Hunig T. Expression of cell interaction molecules by immature rat thymocytes during passage through the CD4+8+ compartment: developmental regulation and induction by T cell receptor engagement of CD2, CD5, CD28, CD11a, CD44 and CD53. Eur J Immunol. 1995; 25(2):328-332. (Biology).
10. Pieters RH, Punt P, Bol M, van Dijken JM, Seinen W, Penninks AH. The thymus atrophy inducing organotin compound DBTC stimulates TcR alpha beta-CD3 signalling in immature rat thymocytes. Biochem Biophys Res Commun. 1995; 214(2):552-558. (Biology).
11. Wang M, Qu X, Stepkowski SM, Chou TC, Kahan BD. Beneficial effect of graft perfusion with anti-T cell receptor monoclonal antibodies on survival of small bowel allografts in rat recipients treated with brequinar alone or in combination with cyclosporine and sirolimus. Transplantation. 1996; 61(3):458-464. (Biology).
12. Yoshino S, Cleland LG, Mayrhofer G, Brown RR, Schwab JH. Prevention of chronic erosive streptococcal cell wall-induced arthritis in rats by treatment with a monoclonal antibody against the T cell antigen receptor alpha beta. J Immunol. 1991; 146(12):4187-4189. (Biology).
13. Yoshino S, Cleland LG. Depletion of alpha/beta T cells by a monoclonal antibody against the alpha/beta T cell receptor suppresses established adjuvant arthritis, but not established collagen-induced arthritis in rats. J Exp Med. 1992; 175(4):907-915. (Biology).
参考图片
Flow cytometric analysis of αβ TCR expression in rat spleen and thymus. Lewis splenocytes were simultaneously stained with FITC Mouse Anti-Rat CD3 (Cat. No. 559975/554832, left panels) and PE Mouse Anti-Rat αβ T-Cell Receptor (Cat. No. 554914; bottom left panel). Lewis thymocytes were stained with PE Mouse Anti-Rat αβ T-Cell Receptor (bottom right panel) or unstained (top right panel). Fluorescent histograms and two-color contour plots were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
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